Microarrays and unbiased next-generation sequencing — moving towards clinically actionable diagnostic assays for pathogens
Charles Chiu, Assistant Professor UCSF School of Medicine
February 13, 2014 6 pm (Meet and greet with food at 5:30 pm)
Blum Hall B100
Microarrays and unbiased next-generation sequencing (NGS) technology enables the detection of novel or uncommon pathogens directly from clinical samples, but its routine implementation in clinical and public health settings has been hindered by issues of cost, turnaround time, and bioinformatics analysis of complex datasets. Here we will describe the use of the technology in the clinical laboratory to validate novel, ultra-sensitive assays that have the potential to transform infectious diseases. This talk will focus on the development and validationof a broad-based microarray assay for the detection of tickborne pathogens (TickChip), and on the implementation of an unbiased NGS approach for diagnosis of infectious diseases. In particular, actionable diagnoses were obtained using NGS for recent cases of fulminant meningoencephalitis in two critically ill patients.
Charles Chiu, Assistant Professor UCSF School of Medicine
February 13, 2014 6 pm (Meet and greet with food at 5:30 pm)
Blum Hall B100
Microarrays and unbiased next-generation sequencing (NGS) technology enables the detection of novel or uncommon pathogens directly from clinical samples, but its routine implementation in clinical and public health settings has been hindered by issues of cost, turnaround time, and bioinformatics analysis of complex datasets. Here we will describe the use of the technology in the clinical laboratory to validate novel, ultra-sensitive assays that have the potential to transform infectious diseases. This talk will focus on the development and validationof a broad-based microarray assay for the detection of tickborne pathogens (TickChip), and on the implementation of an unbiased NGS approach for diagnosis of infectious diseases. In particular, actionable diagnoses were obtained using NGS for recent cases of fulminant meningoencephalitis in two critically ill patients.